![]() Since their initial discovery circRNAs have been developed for various uses. An analysis of circRNA and their associated linear mRNAs revealed that the circRNA isoforms were highly stable, with transcript half-lives exceeding 48 hours, while the associated linear transcripts exhibited half-lives of less than 20 hours (Jeck et al., RNA (2013) 19:141-157 herein incorporated by reference in its entirety). Nature (2013) 495:333-338 herein incorporated by reference in its entirety). Recent studies have confirmed that circRNA is resistant to digestion with RNase R exonuclease and turns over more slowly than its counterpart linear RNA in vivo (Memczak et al. With its 5′ and 3′ ends joined together, circRNA has no free ends and has extraordinary long half-life (Harland & Misher, Development (1988) 102:837-852 herein incorporated by reference in its entirety). BACKGROUND OF THE INVENTIONĬircular RNA was first discovered in 1979 by electron microscope (Hsu et al., Nature (1979) 280:339-340 herein incorporated by reference in its entirety). The invention relates to compositions, methods, processes, kits and devices for the design, preparation, manufacture and/or formulation of single stranded circular polynucleotides (circP). The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety. The Sequence Listing is provided as a file entitled M136SL.txt created on Jul. ![]() ![]() ![]() The present application is being filed along with a Sequence Listing in electronic format. 3, 2014, entitled Circular Polynucleotides the contents of each of which are herein incorporated by reference in its entirety. 16, 2014, entitled Circular Polynucleotides, and to U.S. ![]()
0 Comments
Leave a Reply. |