This globally elevated transcriptional state has been proposed to occur in several contexts of embryonic development. These studies revealed that PGCs express a pluripotency program with high similarity to that of Embryonic Stem (ES) cells, along with unique modules specific to PGCs related to migration and sexual differentiation.ĮS cells can exist in a state of hypertranscription, which involves a global nascent amplification of most of the transcriptome ( Efroni et al., 2008 Guzman-Ayala et al., 2015 Percharde et al., 2017). In parallel, several groups, including our own, have investigated the transcriptional profile of PGCs. Significant insights have been gained into the unique chromatin reprogramming that PGCs undergo during their development. PGC development is accompanied by a remarkable level of epigenetic reprogramming, such as genome-wide DNA demethylation including the removal of genomic imprints, widespread changes to histone modifications and variants, and X-chromosome reactivation in females. From E7.75-E10.5, these cells migrate to the developing gonads where they proliferate and, from E12.5, commence sexual differentiation ( Tam and Snow, 1981 Western et al., 2008). In mice, PGCs arise from a small group of epiblast cells that are first identifiable at embryonic day (E)7.25. Primordial germ cells (PGCs), the embryonic precursors to mature oocytes and spermatozoa, are thus essential for ontogeny and species survival. The germline carries on to the next generation both developmental totipotency as well as genetic and epigenetic predispositions to disease. Or use the native HyperTRANSCRIBE Document (.htd) file as a source file in Researchware’s qualitative analysis software, HyperRESEARCH. When you’re finished transcribing, choose File ↠ Export to save your transcription as plain text, RTF (Rich Text Format), or SBV (YouTube subtitles and captions format). Enter your transcript in the right half of the transcription window as you play the media file.ĥ.
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